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Senin, 16 Mei 2011

What is disinfectant?

Disinfectants are substances that are applied to non-living objects to destroy microorganisms that are living on the objects.Disinfection does not necessarily kill all microorganisms, especially nonresistant bacterial spores ; it is less effective than sterilisation , which is an extreme physical and/or chemical process that kills all types of life.Disinfectants are different from other antimicrobial agents such as antibiotics , which destroy microorganisms within the body, and antiseptics , which destroy microorganisms on living tissue Disinfectants are also different from biocides — the latter are intended to destroy all forms of life, not just microorganisms.
However, ideal disinfectants do not exist. Most disinfectants are also, by nature, potentially harmful (even toxic ) to humans or animals. Most modern household disinfectants contain Bitrex , an exceptionally bitter substance added to discourage ingestion, as a safety measure. Those that are used indoors should never be mixed with other cleaning products as chemical reactions can occur.
The choice of disinfectant to be used depends on the particular situation. Some disinfectants have a wide spectrum (kill many different types of microorganisms), while others kill a smaller range of disease-causing organisms but are preferred for other properties (they may be non-corrosive, non-toxic, or inexpensive).
In this experiment the sample used was handsanitizer with brand A and D.
The purpose of this experiment (disinfectant) is to investigate the influence of disinfectants on the growth of bacteria.
Tools and materials used include:
- Cotton stick sterile
- Samples of bacteria
- Media NA plate
- Filter paper
- Tweezers
- Disinfectants
- Incubator

How it works as follows:
- Enter a sterile cotton stick into the bacterial samples
- Perform smoothing on NA medium plate using a sterile cotton stick
- Incubation 15 minutes at 37 ° C incubator with
- Make 2 pieces of dots on the media plate NA
- Sterilize tweezers, grab the filter paper with tweezers and then wet the paper filter with a disinfectant A
- Put on the first point
- Do the same thing in disinfectant D, then place on the second point
- Incubation 37o C for 24 hours
- Read the results
Results obtained, the radical zone disinfectant A were higher than zone D radicals disinfectant, so bottom line, is more effective against bacterial samples A comparison disinfectant disinfectant D

Disinfectants work on disease-causing bacteria in a variety of ways,
depending on the type of organism, and the kind of disinfectant. Disinfectants differ from antiseptics in that disinfectants are not meant for using on skin. There are 7 major disinfecting chemicals, some of which have a broad spectrum, and some are more specific in the
organisms they target:
1) chlorine,
2) ethyl alchol,
3) glutaraldehyde,
4) iodophors and iodines,
5) isopropyl alcohol,
6) phenolics
7) quaternary ammonium compounds
Some bacteria have outer capsules or spores, which act as protective elements. Other bacteria have cell walls composed of thick lipid
layers. Because of these capsules, lipid layers and spores, not all
disinfectants are effective on all bacterial species. Each
disinfectant kills bacteria in its own way, so to speak. The
effectiveness of each disinfectant is based on the concentration of
the disinfectant solution, and the length of time it is left on the
item to be cleaned.

Other disinfectants kill by coagulating (clotting) the bacterial cell
enzymes, needed for cell function. Chlorine kills by oxidizing sulphydryl groups in bacterial enzymes.

Ethanol and alcohol work by denaturing the bacterial cell proteins,
but not highly recommended because they evaporate quickly. Iodine is Yodium
effective on bacteria like the tuberculosis bacteria and bacteria with
like bacillus subtilus.

Phenol is a corrosive disinfectant, inactivating cell enzymes, and
causing the bacterial cell wall to rupture and leak. Phenol is very bacteriostatic (prevents future bacterial growth)

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